Process for rosetting human B lymphocytes with Rhesus monkey ery

Details Process for rosetting human B lymphocytes with Rhesus monkey ery Process for rosetting human B lymphocytes with Rhesus monkey ery

1979-04-05

1980-03-11

Rosen, Sam

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

Inactivation or attenuation; producing viral subunits

424 12, C12N 704, A61K 3900

Patent

active

041929179

ABSTRACT:
Human peripheral blood lymphocytes are stimulated with either a polyclonal activator or an antigen which gives rise to immunologically activated B lymphocytes. These activated B lymphocytes are separated from non-activated B lymphocytes by relying on the fact that activated B lymphocytes possess a surface marker; the ability to rosette Rhesus monkey lymphocytes (RhMRBC), not found on their non-activated counterparts. Separation is achieved by rosetting with RhMRBC and subsequent density centrifugation of cells.

REFERENCES:
Barrett-Textbook of Immunology 2nd edit. (1974), p. 54.

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