Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1999-01-28
2000-08-08
Guzo, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
530350, 530358, 536 231, 536 243, C12Q 168, C07K 14005, C07H 2104
Patent
active
061000396
ABSTRACT:
Described is a process for detecting reverse transcriptase activity and, thereby, reverse transcriptase inhibitors using fluorescence polarization, comprising, mixing a DNA primer with an RNA template. Then forming an RNA/DNA duplex utilizing the reverse transcriptase and removing the RNA from the RNA/DNA duplex to form single-stranded DNA. Finally, adding a fluorescent-labeled oligonucleotide complementary to the single-stranded DNA for hybridizing to the single-stranded DNA; and, measuring the fluorescence polarization.
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Bolger Randall E.
Burke Thomas J.
Lenoch Francis J.
Guzo David
Johnson Mark K.
Panvera, Inc.
Shuman Jon
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