Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-08-25
1997-10-21
Jones, W. Gary
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 536 243, C12P 1934, C12Q 168, C07H 2104
Patent
active
056795530
ABSTRACT:
A process for making the product of a nucleic acid amplification reaction, which amplification reaction employs one or more primer pairs, incapable of being a target for further amplification comprising: contacting the amplified product with an oligonucleotide capable of hybridizing to a stretch a nucleotides of the amplified product, said stretch being situated between the hybridisation sites of a pair of primers, under conditions which allow formation of a hybridisation complex between the oligonucleotide and the amplified product to occur, said oligonucleotide being modified in such a way that it.protects the double stranded part of the hybridisation complex hybridized to the oligonucleotide from degradation, and subjecting the hybridisation complex to a degradative treatment under circumstances such that at least the part of the hybridisation complex capable of hybridizing to the primers is degraded.
An assay for diagnosing the presence of a nucleic acid sequence in a sample comprising carrying out the above-mentioned method and a kit for carrying out the above-mentioned method are also disclosed.
REFERENCES:
patent: 5273890 (1993-12-01), Steinman
J. Versailles et al., PCR Methods and Applications, 3:3:151-158, Dec. 1993.
Ullu et al, "2'-O-methyl RNA oligonucleotides identify two functional elements in the trypanosome spliced leader ribonucleoprotein particle", J. Biol. Chem. 268(18):13068-13073 Jun. 1993.
Wasserman et al, "Structural analyses of the 7SK ribonucleoprotein (RNP), the most abundant human small RNP of unknown function", Mol. Cell. Biol. 11(7):3432-3445 Jul. 1991.
Stratagene catalog, p. 39 1988.
Yap et al, "False positives and contamination in PCR", in PCR Technology: Current Innovations, CRC Press, Boca Raton, Fl pp. 249-258 1994.
DeFilippes, "Decontaminating the polymerase chain reaction", Biotechniques 10(1):26, 28, 30 1991.
Zhu et al, "The use of exonuclease III for polymerase chain reaction sterilization", Nucleic Acids Res. 19(9): 2511 1991.
Hod, "A simplified ribonuclease protection assay", Biotechniques 13(6):852-853 1992.
Saccomanno et al, "A faster ribonuclease protection assay", Biotechniques 13(6):847-849 1992.
Yang et al, "Application of the polymerase chain reaction to the ribonuclease protection assay", Biotechniques 13(6):922-927 1992.
Schukkink Adriana Frederieke
Van Gemen Bob
Akzo Nobel N.V.
Fredman Jeffrey
Gormley Mary E.
Jones W. Gary
LandOfFree
Process for rendering a nucleic acid amplication reaction produc does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for rendering a nucleic acid amplication reaction produc, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for rendering a nucleic acid amplication reaction produc will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1006081