Process for renaturation of recombinant, disulfide...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

Reexamination Certificate

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C530S408000, C530S410000

Reexamination Certificate

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07893210

ABSTRACT:
A method for renaturation of proteins comprising adding to a solution of denatured, chemically modified or reduced proteins a refolding buffer containing a primary, secondary or tertiary amine. Said method has been applied, for example, to interleukin-4 and bovine pancreatic trypsin inhibitor (BPTI), which were previously (i) solubilized in the presence of guanidinium hydrochloride as chaotronic agent, and (ii) subjected to sulfitolysis.

REFERENCES:
patent: 5453363 (1995-09-01), Rudolph et al.
patent: 5563057 (1996-10-01), Gellman et al.
patent: 5593865 (1997-01-01), Rudolph et al.
patent: 5721111 (1998-02-01), Unno et al.
patent: 5723118 (1998-03-01), Sebald
patent: 5728804 (1998-03-01), Sharma et al.
patent: 5756672 (1998-05-01), Builder et al.
patent: 2002/0052026 (2002-05-01), Vicik
patent: 2004/0018586 (2004-01-01), Rosendahl et al.
patent: 0613499 (1994-09-01), None
patent: 1022339 (2000-07-01), None
patent: 8901046 (1989-02-01), None
patent: 9640784 (1996-12-01), None
patent: 9933988 (1999-07-01), None
patent: 0073460 (2000-12-01), None
patent: 0187925 (2001-11-01), None
Novagen Protein Refolding Kit reference sheets 1998: 9 pages.
De Bernardez Clark, Eliana, “Refolding of Recombinant Proteins,”Current Opinion in Biotechnology, 9: 157-163 (1998).
Lilie, et al., “Advances in Refolding of Proteins Produced inE. coli,” Current Opinion in Biotechnology, 9: 497-501 (1998).
Daugherty, et al., “Artificial Chaperone-assisted Refolding of Citrate Synthase,”J. of Biological Chemistry, 273 (51): 33961-33971 (1998).
Rozema, et al., “Artificial Chaperone-assisted Refolding of Carbonic Anhydrase B,”J. of Biological Chemistry, 271 (7): 3478-3487 (1996).
Creighton, et al., “On the Biosynthesis of Bovine Pancreatic Trypsin Inhibitor (BPTI): Structure, Processing, Folding and Disulphide Bond Formation of the Precursor in Vitro and in Microsomes,” J. Mol. Biol, 232, 1176-1196 (1993).

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