Process for removing nucleic acids and purine bases from gelatin

Details Process for removing nucleic acids and purine bases from gelatin Process for removing nucleic acids and purine bases from gelatin

1987-06-12

1988-09-20

Therkorn, Ernest G.

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

2101982, 2105021, 530355, 530417, B01D 1508

Patent

active

047723971

ABSTRACT:
The invention relates to a process for removing nucleic acids and purine bases from gelatine by
(a) adding cyclodextrin bead polymer having a particle size of 20 to 600 .mu., a cyclodextrin content of 30 to 70% and a polyvinyl alcohol content of 0.3 to 0.5% to the gelatin solution in a weight ratio of 1:1 to 10:1 calculated on the amount of the gelatin then filtering off the cyclodextrin polymer or
(b) passing the gelatin solution through a cyclodextrin bead polymer column, whereby the amount of the gelatin is 5 to 50% of the weight of the column charge, then eluting the nucleic acids and the purine bases from the cyclodextrin bead polymer.

REFERENCES:
patent: 3472835 (1969-10-01), Buckler
patent: 3649456 (1972-03-01), Benneville
patent: 4199450 (1980-04-01), Dulout
patent: 4360590 (1982-11-01), Tomka
patent: 4426292 (1984-01-01), Wernick
patent: 4515714 (1985-05-01), Kawahara
On the Method for the Quantitative Separation of Nucleic Acid in Native Form From Photographic Gelatin, Masuda, The Journal of Photographic Science, vol. 30, 1982, pp. 124-128.

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