Process for removing bacterial endotoxin from gram-negative poly

Details Process for removing bacterial endotoxin from gram-negative poly Process for removing bacterial endotoxin from gram-negative poly

1990-10-09

1991-08-13

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

210601, 210616, 210631, 424 88, 424 92, 435170, 435262, 435280, 435800, 435803, 435851, 536 11, C12P, C12P, C12P 104, C12R 121

Patent

active

050396102

ABSTRACT:
A process for removing endotoxin from Gram-negative polysaccharides such as polyribosylribitol phosphate by solubilizing polysaccharide-containing powder derived from Gram-negative bacteria fermentation broth to provide a divalent counter ion for endotoxin and adding alcohol incrementally to induce lipopolysaccharide precipition, and mixing material resulting from the alcohol addition with a nonionic resin, a detergent and a chelating agent.

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