Process for regenerating cereals

Details Process for regenerating cereals Process for regenerating cereals

1984-09-07

1987-05-19

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435241, 800 1, C12N 500, C12N 502

Patent

active

046668446

ABSTRACT:
The present invention relates to the regeneration of cereals including barley, corn, wheat, rice and sorghum. The process comprises the steps of:
(a) culturing tissue obtained from a cereal plant on an induction medium comprising mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure callus formation,
(b) culturing the callus on a series of media, said series comprising at least one medium and each medium comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to insure differentiating to plantlets having shoots and roots at the completion of the series, and
(c) culturing the plantlets on an establishment medium comprising mineral salts, vitamins and sucrose, whereby the plantlets are established so that they can be transplanted to soil.

REFERENCES:
Maddock et al., 1983, J. Exp. Bot., v 34, 915-26, (Ref. #20).
Evans et al., 1981, "Growth and Behavior of Cell Cultures: Embryogenesis and Organogenesis" in Plant Tissue Culture, Thorpe, ed. Academic Press, pp. 45-113.
Conger (ed.), 1981, Cloning Agricultural Plants via in vitro Techniques, p. 55.
The Yearbook of Agriculture 1961 Congressional Document, No. 29, 2 plates.

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