Process for reduction of the matrix effect in a fructosamine det

Details Process for reduction of the matrix effect in a fructosamine det Process for reduction of the matrix effect in a fructosamine det

1987-06-19

1992-10-20

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 10, 435 27, 435 28, 436 87, 436 88, 436 95, 436175, 436825, 436904, C12Q 100, C12Q 128, C12Q 130

Patent

active

051569477

ABSTRACT:
This invention teaches a process for reducing protein matrix effects in assays for serum fructosamine. Blood or blood derived samples are used, and one adds two reagents, one of which reduces interference caused by non-specific reducing substances, the other of which eliminates turbidity. Incubation follows, and then the pH of the sample is adjusted and color forming reagent is added. In one embodiment, the incubation time is only 1-15 minutes. In another embodiment, the first reagent contains peroxidase.

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Johnson et al, Clin. Chim. Acta, vol. 127, pp. 87-95, 1982.

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