Process for reducing the salt concentration in a biomass suspens

Details Process for reducing the salt concentration in a biomass suspens Process for reducing the salt concentration in a biomass suspens

1990-03-15

1992-10-13

Cashion, Jr., Merrell C.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Utilizing media containing lower alkanol

435183, 435255, 435256, 435261, 210634, 210767, 530424, C07K 300, C07K 302

Patent

active

051550407

ABSTRACT:
Alcohol is suitable for the extraction of salt from biomass-containing suspensions, in particular from the salt-containing lower phase of an aqueous 2-phase extraction of intracellular proteins after cell disruption, with the formation of a salt-containing alcoholic upper phase. It is possible to use ethanol, propanol, isopropanol or tert.-butanol, but especially ethanol, as the alcohol. The upper phase is separated from the lower phase by disk separators or decanters. The extraction is carried out, in particular, as a countercurrent extraction in at least three stages, and uses 10 to 30% by weight salt-containing suspension or liquid with 30 to 50% by weight alcohol, in particular ethanol, remainder water. The alcohol is removed from the resulting salt-rich upper phase by evaporation, and the salt solution is recycled where appropriate after further concentration to obtain proteins.

REFERENCES:
patent: 3844893 (1974-10-01), Hitzman
patent: 4816407 (1989-03-01), Matson
T. Maniatis, et al., "Molecular Cloning a Laboratory Manual", Cold Spring Harbor Laboratory, pp. 461-462, (1982).

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