Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1992-04-16
1993-06-01
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435225, 435815, C12N 964
Patent
active
052159086
ABSTRACT:
This invention provides a method of recovering and purifying chymosin from fermentation used for microbial production of chymosin. The fermentation mixture is filtered to provide a filtrate containing the chymosin. The filtrate is first prepared by either adjusting it to a low pH, such as about 2 and/or adjusting the salt concentration of the filtrate to at least about 2M. The aqueous filtrate mixture is contacted with a phenyl-sepharose resin, which selectively binds the chymosin from the filtrate. The filtrate remaining after the contact with the phenyl-sepharose resin is discarded, and the phenyl-sepharose resin column is eluted with water or dilute salt solution to obtain substantially pure chymosin in a single elution step.
REFERENCES:
patent: 2339931 (1944-01-01), Keil
patent: 3281332 (1966-10-01), Munns et al.
patent: 3834990 (1974-09-01), Werle et al.
patent: 4016039 (1977-04-01), Schreiber
patent: 4145406 (1979-03-01), Schick et al.
patent: 4250084 (1981-02-01), Trainin
patent: 4259319 (1981-03-01), Umezawa et al.
patent: 4264738 (1981-04-01), Stepanov et al.
patent: 4332717 (1982-06-01), Kanaoka et al.
patent: 4461833 (1984-07-01), Gordon
patent: 4471053 (1984-09-01), Comi et al.
patent: 4568488 (1986-02-01), Lee-Huang
patent: 4666843 (1987-05-01), Subramanian
patent: 4675384 (1987-06-01), Dromard et al.
patent: 4684723 (1987-08-01), Dove et al.
patent: 4687740 (1987-08-01), Waite
patent: 4710566 (1987-12-01), Kozarich et al.
patent: 4721673 (1988-01-01), Uren et al.
patent: 4722999 (1988-02-01), Handschumacher et al.
patent: 4743551 (1988-05-01), Subramanian
patent: 4745063 (1988-05-01), Birschbach
patent: 4752578 (1988-06-01), Moore et al.
patent: 4757008 (1988-07-01), Reverman
Biotechnology Applications and Research (1985), Cheremisinoff, et al., eds., Technomic Pub., pp. 549-552.
Pharmacia Biotechnology Products Catalog 86 (1986) p. 93.
Franke, et al., J. of Ind. Microbiology, Suppl. No. 3 pp. 43-57.
Heinsohn Henry G.
Murphy Matthew B.
Genencor International Inc.
Patterson Jr. Charles L.
LandOfFree
Process for recovery and purification of chymosin does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for recovery and purification of chymosin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for recovery and purification of chymosin will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1813958