Process for recovering refractile bodies containing heterologous

Details Process for recovering refractile bodies containing heterologous Process for recovering refractile bodies containing heterologous

1991-11-25

1993-09-28

Russel, Jeffrey E.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

435 691, 435 695, 435 6951, 435 6952, 530351, 530412, 530427, C07K 312, C07K 326

Patent

active

052487695

ABSTRACT:
A refractile material containing a heterologous protein is recovered from a host microorganism cell culture transformed to produce the protein. One recovery process involves first reducing the ionic strength of the culture medium prior to disruption to a level effective in preventing reaggregation of cellular debris with refractile material after disruption, disrupting the desalted culture, optionally adding material to the disruptate to create a density or viscosity gradient and separating the refractile material from the cellular debris by high-speed centrifugation. Preferably the salt removal step is carried out by diafiltration and the heterologous protein comprises recombinant M-CSF, IL-2 or IFN-.beta..

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