Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1986-03-25
1988-05-31
Kight, John
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530351, 530414, 530416, 530417, 530422, 530424, 530825, 435 68, 435 70, 424 85, C07K 312, C07K 328, A61K 4502
Patent
active
047482346
ABSTRACT:
A refractile material containing a heterologous protein is recovered from a host microorganism cell culture transformed to produce the protein. One recovery process involves disrupting the cell wall and membrane of the host cell, removing greater than 99% by weight of the salts from the disruptate, redisrupting the desalted disruptate, adding a material to the disruptate to create a density or viscosity gradient in the liquid within the disruptate, and separating the refractile material from the cellular debris by high-speed centrifugation. Another version of such a recovery process comprises the further steps of solubilizing the refractile material under reducing conditions, organically extracting the solubilized refractile material, and isolating said refractile material from the extractant.
Preferably the protein is recombinant IL-2 or IFN-.beta. and the salt removal step is carried out by diafiltration.
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Dorin Glenn
Hanisch Wolfgang H.
Lin Leo S.
Cetus Corporation
Draper Garnette D.
Halluin Albert P.
Kight John
Lauder Leona L.
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