Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide
Patent
1990-11-05
1992-06-23
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using a micro-organism to make a protein or polypeptide
435183, 435206, 530424, C12P 2100, C12N 900, C12N 936, C07K 302
Patent
active
051242567
ABSTRACT:
The invention relates to a process for releasing into aqueous medium polypeptides produced by yeasts and localized at least partially within the periplasmic space thereof without breaking the yeast cell. The process involves treating the yeasts in aqueous medium with a neutral water-soluble mineral salt and a non-ionic water-soluble polyethoxylated alkylphenol surfactant having an HLB of between 8 and 15.
REFERENCES:
patent: 3917510 (1975-11-01), Kitamura
patent: 4683293 (1987-07-01), Craig
patent: 4683294 (1987-07-01), Van Wijnendaele
Muraki, M. et al., Agric. Biol. Chem., 49(a): 2829-2831 (Sep. 1985).
Teich et al., Chem. Abstracts 92(3): 196398c, p. 518 (1980).
Yasudo et al. Chem. Abstracts 91(1):3933w, (1979).
Tuite et al. EMBO J. 1(5): 603-608 (1982).
Scopes, Protein Purification, Springer-Verlag, New York, .COPYRGT. 1982, pp. 36 and 37.
Hitzeman et al.; Nucleic Acids Research, 11(9):2745-2763, (1983).
Ota, Y et al., Agric. Biol. Chem 46(12):2885-2893 (82) Purification and some Properties of Cell-Bound Lipase.
Schwencke et al., Eur. J. Biochem 21:137-143 (71) The Release of Extracellular Enzymes from Yeast by "Osmotic Shock".
G. L. Stetler et al. BIO/TECHNOLOGY, vol. 7, pp. 55 to 60, Jan. 1989 "Secretion of Active, Full-and Half-Length Human Secretory Leukocyte Protease Inhibitor by Saccharomyces Cerevisiae".
Crahay Jacques
Delcour Jean M. A. G.
Hanotier Jacques D. V.
Abokhair John K.
Cheairs M. Norwood
Labofina S.A.
Parkhurst Roger W.
Robinson Douglas W.
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