Process for recovering microbially produced interleukin-2 and pu

Details Process for recovering microbially produced interleukin-2 and pu Process for recovering microbially produced interleukin-2 and pu

1984-03-28

1986-02-11

Kight, John

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

2601125R, 435 68, 4351723, C07K 312, C12D 2102

Patent

active

045697906

ABSTRACT:
A process for recovering microbially produced IL-2 in a highly pure form from the cellular material of the microorganisms that produced it comprising: disrupting the cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC.

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