Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Patent
1988-03-25
1990-06-05
Draper, Garnette D.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
530402, 530408, 530409, 530410, 530412, 530416, 530417, 530422, 530424, 530825, 435 6952, C07K 328
Patent
active
049315439
ABSTRACT:
A process for recovering substantially pure rIL-2 from transformed microorganisms in which the cells are disrupted, impure insoluble rIL-2 is separated from the bulk of the cellular components, the separated impure rIL-2 is solubilized and partially purified in a reduced form, the solubilized rIL-2 is oxidized, the oxidized rIL-2 is purified to clinically acceptable levels, and the oxidized, purified IL-2 is denatured by placing it into a solution of a chaotropic agent, solids are removed from the solution and rIL-2 is renatured from the solution.
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Halenbeck Robert
Kunitani Michael
Smith Flint
Cetus Corporation
Draper Garnette D.
Halluin Albert P.
McGarrigle Philip L.
Murphy Lisabeth F.
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