Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1987-05-11
1990-10-09
Hazel, Blondel
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
424 856, 530351, C12P 2100, A61K 4502, C07K 1526
Patent
active
049619691
ABSTRACT:
An approved procedure for the purification and renaturation of biologically active, bacterially produced IFN-.beta. is described. The partially purified material obtained by solubilization of isolated refractile bodies from the recombinant cells is treated to obtain reduction of the protein in the presence of a chaotropic environment and then oxidized after removal of the reducing agent. However, the chaotropic environment is retained during the oxidation. Upon removal of the chaotropic environment, a solubilizing additive is supplied to maintain the IFN-.beta. in solution. Further purification by conventional means may also be effected.
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Hershenson Susan
Shaked Ze'Ev
Thomson Jody
Cetus Corporation
Halluin Albert P.
Hazel Blondel
Murphy Lisabeth E.
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