Drug – bio-affecting and body treating compositions – Lymphokine
Patent
1982-09-27
1985-07-02
Hazel, Blondel
Drug, bio-affecting and body treating compositions
Lymphokine
260112R, 435 68, A61K 4502, C07G 700, C12P 2100
Patent
active
045267820
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
This invention relates to a process for recovering on an industrial scale an interferon produced by induced cells of human origin.
BACKGROUND ART
Interferon is a certain type of glycoprotein induced in human and other animal cells by viral or other stimuli. The interferon has an inhibitory action for the growth of virus, bacteria, or protozoa in cells. In order to apply it in man as a medicine, it is necessary to obtain the interferon produced in human cells because of its species specific. To obtain large quantities of human interferon, it is necessary to collect a large quantity of human lymphocytes, human fibroblasts, or human lymphoblasts, etc. then to stimulate them by suitable means to produce the interferon, and to recover the formed interferon in a high yield.
The conventional recovering methods, however, have such defects that they are too complicated in the procedure and are too time-consuming to be carried out on a commercial scale, and further they have an additional disadvantage of low material yield.
DISCLOSURE OF INVENTION
The present inventors have conducted researches for many years on the method of recovering interferon in a good yield by simple operations, and found that a silicic acid-containing material such as bentonite, acid clay, etc. adsorbs interferon specifically. They further continued studies on the elution of interferon from the adsorbent and found that interferon is specifically eluted from the adsorbent when an aqueous solution containing a nonionic surfactant or containing in addition acriflavines is applied. Based on the new knowledge, they have accomplished the present invention, that is, they were successful in recovering interferon in a good yield on an industrial scale by simple operations.
This invention provides a process for recovering interferon, which comprises contacting a silicic acid-containing material with an aqueous solution containing interferon induced and produced from cells of human origin, to adsorb the interferon on the composition and thereafter eluting the adsorbed interferon with an aqueous solution containing a nonionic surfactant or which may additionally contain an acriflavine.
BEST MODE FOR CARRYING OUT THE INVENTION
Any of known interferon-producing cells such as leukocytes; lymphocytes; reticuloendothelial cells such as those of the peritoneal cavity, lung, and spleen; cultured human lymphoblast-like cells, can be used as the cells of human origin in this invention. However, preferred in view of productivity are human leukocytes and cultured human lymphoblast-like cells from the productivity point of view, and among the latter cells the Namalwa strain is particularly preferred. The Namalwa strain is an established cell line selected from 20 established lymphocytes originated from Burkitt's lymphoma patients or leukemia patients, and is known at present as a cell most productive of interferon [Intern. J. Cancer, 11, No. 327 (1973) and J. Clinical Microbiol, 1, No. 1 (1975)].
The acid (SiO.sub.2) containing material, usable in this invention includes bentonite, acid clay, kaolin, magnesium aluminosilicate, and compounds corresponding thereto. The concentration of the silic acid-containing composition used is within the range of 0.001-4.0 W/V%, usually 0.01-1.0 W/V%, based on the interferon-containing aqueous solution. The contact of the silicic acid-containing composition with the interferon-containing aqueous solution is usually effected batchwise by adding the former to the latter. The contact is preferably effected at a pH of 5-9 with stirring. The temperature and period of contact may be room temperature and 1-5 hours, respectively. By such a contacting operation interferon is adsorbed on the silicic acid-containing composition, and this adsorption is extremely specific.
The elution of the adsorbed interferon is effected with an aqueous solution containing a nonionic surfactant, preferably with an aqueous solution containing both a nonionic surfactant and an acriflavine, after the silicic acid-containing composition
REFERENCES:
Chemical Abstracts, vol. 95, Abst. No. 12751v, 1981.
Chemical Abstracts, vol. 94, Abst. No. 180664s, 1981.
Chemical Abstracts, vol. 94, Abst. No. 180663r, 1981.
Arimura Hirofumi
Funakoshi Satoshi
Morise Hiroshi
Suyama Tadakazu
Uemura Yahiro
Hazel Blondel
The Green Cross Corporation
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