Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1980-09-15
1982-07-06
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435105, 435166, 435167, 435267, 435813, C12P 1914, C12P 1902, C12P 500, C12P 502
Patent
active
043383998
ABSTRACT:
A process for enzymatically converting whole plant biomass containing hydrocarbon-containing laticifers to soluble sugars and recovering hydrocarbons in increased yields which comprises hydrolyzing whole plant cellulosic material in the presence of enzymes, particularly cellulase, hemicellulase, and pectinase, to produce a hydrocarbon product and recovering from the hydrolysis products a major proportion of the cellulase, hemicellulase and pectinase enzymes for reuse. At least some portion of the required make-up of cellulase, hemicellulase and pectinase enzymes is produced in a two-stage operation wherein, in the first stage, a portion of the output sugar solution is used to grow enzyme secreting microorganisms selected from the group consisting of cellulase-secreting microorganisms, hemicellulase-secreting microorganisms, pectinase-secreting microorganisms, and mixtures thereof, and in the second stage, cellulase, hemicellulase and pectinase enzyme formation is induced in the microorganism-containing culture medium by the addition of an appropriate inducer such as biomass. The cellulase, hemicellulase and pectinase enzymes are then recycled for use in the hydrolysis reaction.
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Nielson, et al. "Plant Crops as a Source of Fuel and Hydrocarbon-like Materials", Science, vol. 198, (1977), pp. 942-944.
Buchanan, et al. "Hydrocarbon--and Rubber-Producing Crops Evaluation of 100 U.S. Plant Species", Chem. Abstracts, vol. 90, (1979), p. 308, abs. No. 3150t.
Dzadzic Peter M.
Price Michael C.
Shih Chien-Cheng J.
Weil Thomas A.
Clarke William C.
Magidson William H.
McClain William T.
Standard Oil Company (Indiana)
Wiseman Thomas G.
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