Process for recovering human IFN-.beta. from a transformed micro

Details Process for recovering human IFN-.beta. from a transformed micro Process for recovering human IFN-.beta. from a transformed micro

1982-03-01

1984-05-22

Wiseman, Thomas G.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

2601125R, 435 68, 435 70, 435811, 424 85, C07G 700, C12P 2100, C12P 2102, A61K 4502

Patent

active

044501030

ABSTRACT:
A process for recovering IFN-.beta. from transformed bacteria comprising: disrupting the cell membranes of the bacteria; solubilizing the IFN-.beta. from the disruptate into an aqueous medium with a solubilizing agent such as sodium dodecyl sulfate; extracting the IFN-.beta. from the aqueous medium with 2-butanol, 2-methyl-butanol, or mixtures thereof under conditions that maintain phase separation between the aqueous meduim and the extractant; and isolating the IFN-.beta. from the extractant such as by precipitating the IFN-.beta. from an aqeous buffer mixture of the extractant by lowering the pH thereof.

REFERENCES:
patent: 4289689 (1981-09-01), Friesen et al.
Laboratory Text in Organic Chemistry, Cason et al., Prentice-Hall, Inc., 1962, pp. 255-269.
Derynck et al.: Nature 287, 193 (1980).
Scandella et al.: Biochemistry 10, 4447 (1971).

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