Process for recovering cellulases

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435813, 435913, C12N 942, C12R 166

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047466111

ABSTRACT:
A cellulosic material is saccharified by cellulases and the resulting saccharified solution is acidified. Subsequently chitosan and/or partially deacetylated chitin are dissolved therein and the obtained solution is alkalified. Thus the cellulases are absorbed by the chitosan and/or partially deacetylated chitin. Further a cellulosic material is saccharified by at least one cellulase originating from a fungus belonging to the genus Aspergillus and at least either chitosan and/or partially deacetylated chitin are added to the resulting saccharified solution to thereby adsorb the cellulase by the chitosan and/or partially deacetylated chitin.

REFERENCES:
patent: 4167447 (1979-09-01), Masri et al.
patent: 4510248 (1985-04-01), Nakanishi et al.
patent: 4552845 (1985-11-01), Reid
Bungay in Enzyme Engineering 7, (N.Y. Academy of Sciences), 1984, pp. 155-157.
Sinitsyn et al. in Applied Biochemistry and Biotechnology, vol. 8, pp. 25-29, (1983).

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