Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1979-09-21
1981-03-03
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
23230B, 424 3, 424 8, 424 12, C12Q 166
Patent
active
042542197
ABSTRACT:
A method of detecting and quantitating the migration of leucocytes in agar gel by (1) fixing the cells; (2) staining them with Ponceau S or a similar dye; (3) destaining; and (4) dissolving the gel by gentle heating in the destaining solution. The stained cells remain adhered to the glass surface of the culture dish, and the cells can be read through a projection microscope or photographed for a permanent record.
REFERENCES:
patent: 3960489 (1976-06-01), Giaever
patent: 3960490 (1976-06-01), Giaever
J. E. Clausen, "Leukocyte Migration Inhibitory Factor", Manual of Clinical Immunology, Rose & Friedman Editors, American Soc. for Microbiology, pp. 100-105 (1976).
Levitt Julian S.
Merck & Co. , Inc.
Perrella Donald J.
Warden Robert J.
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