Process for reactivating purified membrane proteins

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...

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530412, 530413, 530381, C07K 100, C07K 1400

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active

055999093

ABSTRACT:
Processes for reactivating membrane proteins are disclosed. The membrane proteins are reactivated by mixing membrane proteins with phospholipids, in the absence of detergents, at acidic pH, followed by neutralization by buffer addition, or by mixing membrane proteins with phospholipids, in the absence of detergents, at elevated temperature, followed by buffer addition

REFERENCES:
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"Cloning And Expression Of Human Tissue Factor cDNA", Fisher et al., Thrombosis Research 48:89-99 (1987).
"Molecular Cloning of the cDNA for Tissue Factor, the Cellular Receptor for the Initiation of the Coagulation Protease Cascade", Morrissey et al., Cell 50:129-135 (1987).
"Purification of Human Brain Tissue Factor", Broze, Jr., et al., The Journal of Biological Chemistry 260(20):10917-10920 (1985).
"Purification and Characterization of Bovine Tissue Factor", Bach et al., The Journal of Biological Chemistry 256(16):8324-8331 (1981).
"Reconstitution And Lipid Requirements Of Porcine Tissue Thromboplastin", Wijngaards et al., Biochimica et Biophysica Acta 488:161-171 (1977).
Darnell et al., "Molecular Cell Biology", 1986, pp. 59-60, 569-583.
Cantor et al., "Biophysical Chemistry", pp. 287-288.
Derwent Publications Abstract J02188532.

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