Process for reacting substrates with biocatalysts in a 2-phase l

Details Process for reacting substrates with biocatalysts in a 2-phase l Process for reacting substrates with biocatalysts in a 2-phase l

1987-05-29

1989-02-21

Castel, Benoit

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435288, 435316, 422140, C12N 1102, C12P 4100

Patent

active

048064777

ABSTRACT:
A process for reacting substrates with biocatalysts in a two-phase liquid system disposed in a columnar vessel having a top portion with an exit opening and a first filter disposed therein, a bottom portion with an entrance opening and a second filter disposed therein, a feed line connecting the exit and entrance openings, and a pump disposed in the feed line. The process comprises the steps of: stirring the two-phase liquid in the columnar vessel to accelerate sedimentation and minimize accumulation of the biocatalysts in the top portion of the vessel; selectively controlling the temperature of the two-phase liquid within the vessel; drawing, with the pump, a portion of the two-phase liquid through the exit opening and first filter; and passing the drawn-off portion of the two-phase liquid through the feed line and through the entrance opening and second filter into the vessel to enhance miscibility of the individual phases of the two-phase liquid with the vessel.

REFERENCES:
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patent: 4320089 (1982-03-01), Huttlin
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patent: 4589927 (1986-05-01), Allen et al.
patent: 4618418 (1986-10-01), Heijnen et al.
"The Production of the Antiobiotic Patulin in a Three Phase Fluidized Bed Reactor: II. Longevity of the Biocatalyst," The Canadian Journal of Chemical Engineering, vol. 62, Feb., 1984.

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