Process for rapidly enumerating viable entities

Details Process for rapidly enumerating viable entities Process for rapidly enumerating viable entities

1988-04-22

1990-09-25

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435 29, 435 30, 435 32, 435 39, 435177, 435182, C12Q 170, C12Q 102

Patent

active

049593013

ABSTRACT:
A process for rapidly enumerating viable biological entities is disclosed, wherein viability is determined by the critierion of growth of biological entities contained in microdroplets. Alternatively, in some cases, viability is determined by use of vital staining of biological entities contained in microdroplets. The process involves formation of microdroplets, which are very small volume liquid or gel particles, such that some of the microdroplets contain biololgical entities, followed by measurements of biological entities and of microdroplet volumes, such that use of statistical analysis can be used self-consistently to determine the number of viable entities per volume of a sample.

REFERENCES:
patent: 4399219 (1983-08-01), Weaver
patent: 4401755 (1983-08-01), Weaver
patent: 4643968 (1987-02-01), Weaver
patent: 4647536 (1987-03-01), Mosbach et al.
patent: 4649109 (1987-03-01), Perlman
patent: 4801529 (1989-08-01), Perlman
J. C. Weaver Biotechnology and Bioengineering Symp., 17:185-195 (1986).

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