Process for rapid solution synthesis of peptides

Details Process for rapid solution synthesis of peptides Process for rapid solution synthesis of peptides

2003-10-23

2008-10-14

Epperson, Jon D (Department: 1639)

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Synthesis of peptides

C506S027000

Reexamination Certificate

active

07435791

ABSTRACT:
The present invention relates to a process for rapid solution synthesis of a peptide, the process comprising repetitive cycles of steps (a)-(d):(a) a coupling step, using an excess of an activated carboxylic component to acylate an amino component,(b) a quenching step in which a scavenger is used to remove residual activated carboxylic functions, wherein the scavenger may also be used for deprotection of the growing peptide,(c) one or more aqueous extractions and optionally, (d) a separate deprotection step, followed by one or more aqueous extractions, characterised in that the process comprises at least one step (b), referred to as step (b′), in which an amine or a thiol comprising a free anion or a latent anion is used as a scavenger of residual activated carboxylic functions.During the process of this invention the growing peptide need not be isolated until the final peptide sequence has been obtained.This highly efficient process is useful for the production of oligo- and polypeptides of high purity.

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