Process for rapid isolation of high molecular weight DNA

Details Process for rapid isolation of high molecular weight DNA Process for rapid isolation of high molecular weight DNA

1986-09-26

1990-02-13

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Lysis of micro-organism

435 6, 435264, 435267, 435270, C12N 106, C12N 108

Patent

active

049006770

ABSTRACT:
A procedure for isolating high molecular weight nucleic acids utilizing a mixture of lytic enzymes and a chaotropic agent to complete protein denaturation and dissociation from nucleic acids is provided. The nucleic acids so obtained are useful for restriction enzyme analysis and DNA probe hybridization.

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Gross-Bellard et al., European Journal of Biochemistry, 36, 32-38 (1973).
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