Process for purifying urokinase

Details Process for purifying urokinase Process for purifying urokinase

1978-06-01

1980-02-19

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C07G 7026

Patent

active

041893506

ABSTRACT:
A process for purifying urokinase by contacting a crude urokinase-containing solution with a strongly acidic cation exchanger having sulfonic acid groups and adjusted to the NH.sub.4.sup.+ type to adsorb urokinase on said cation exchanger and then eluting urokinase from said exchanger, characterized in that urea is previously added to the crude urokinase-containing solution in such a rate that the final molar concentration of urea in the solution is 3 to 8 M. This process is capable of providing purified urokinase having substantially constant and high specific activity from a crude urokinase-containing solution with a high recovery regardless of purity of the crude urokinase.

REFERENCES:
patent: 3957582 (1976-05-01), Stried et al.
patent: 4025390 (1977-05-01), Urakawa et al.

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