Process for purifying recombinant proteins, and products thereof

Details Process for purifying recombinant proteins, and products thereof Process for purifying recombinant proteins, and products thereof

1986-09-25

1988-03-29

Marantz, Sidney

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 5, 436533, 436534, 436547, 530412, 530826, C07K 300, G01N 33546

Patent

active

047343621

ABSTRACT:
The present invention relates to a process for recovering recombinant protein from refractile inclusion bodies of microorganisms which comprise disrupting cells, recovering refractile bodies containing recombinant protein, solubilizing the refractile bodies with a denaturant, protecting sulfhydryl groups of recombinant proteins, converting cationic amino groups of recombinant proteins to anionic carboxylic acid by derivatization with organic cyclic acid anhydrides, and recovering recombinant protein derivatives.
The present invention also relates to the production of solid supports coated with recombinant protein recovered by the process of the invention, and the use of said devices for the detection and quantification of antibodies to said recombinant proteins in body fluids.

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