Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Albumin
Patent
1995-08-31
1999-10-05
Wax, Robert A.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Albumin
530412, 530413, 530414, 530415, 530416, 530362, 530363, 435 691, 435 696, C07K 116
Patent
active
059626496
ABSTRACT:
The invention provides a process for purifying recombinant human serum albumin (rHSA) by heating a culture medium containing rHSA and the rHSA-producing host cello, feeding said heated solution upwardly into a fluidized bed in which adsorbent particles are suspended to effect contacting with the adsorbent particles and then recovering the adsorbed fraction containing the rHSA, and a composition comprising rHSA which shows a A35D/A280 ratio of below 0.015, when formulated into a 25% solution of said albumin.
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McCreath et al. (1992) J. Chromatog. 597, pp. 189-196.
Burns et al. (1987) Annals of the New York Acad. Sci, vol. 501, pp. 103-107.
Gordon et al. (1990) Biotech. Adv. vol. 8, pp. 741-762.
Biotechnology and Bioengineering, vol. 48, pp. 461-475, 1995, Evans et al., "Countercurrent Gradient Chromatography: A Continuous Focusing Technique."
Annals of the New York Academy of Sciences, 1987, Burns et al., "The Magnetically Stabilized Fluidized Bed as a Biochemical Processing Tool."
Biotechnology Advances, vol. 8, No. 4, pp. 746-762, 1990, Gordon et al., "An Overview Of Continuous Protein Purification Processes."
Journal of Chromatography, 597 (1992), pp. 189-196, McCreath et al., "Novel Affinity Separations Based on Perfluorocarbon Emulsions Use of a Perfluorocarbon Affinity Emulsion for the Purification of Human Serum Albumin From Blood Plasma in a Fluidised Bed."
Noda Munehiro
Ohmura Takao
Sumi Akinori
Yokoyama Kazumasa
Longton Enrique D.
Wax Robert A.
Yoshitomo Pharmaceutical Industries, Ltd.
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