Process for purifying recombinant hepatitis antigens

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

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530412, 530418, 530419, 530422, 530300, 530350, 530824, 530371, 424 89, C07K 326

Patent

active

050119150

ABSTRACT:
Methods of purifying recombinant surface antigen of hepatitis B virus are disclosed. In one protocol, purification is achieved by selective extraction of the antigen from yeast membranes, followed by solubilization with urea and dithiothreitol.

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