Process for purifying nucleoside-5'-diphosphates

Details Process for purifying nucleoside-5'-diphosphates Process for purifying nucleoside-5'-diphosphates

1989-04-07

1992-04-28

Brown, Johnnie R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 27, 536 29, 210656, 210635, C07H 1910, C07H 1920

Patent

active

051091259

ABSTRACT:
This invention is a process for purifying nucleoside-5'-diphosphates containing substituents other than primary amines (hereinafter designated as NDPs) from complex mixtures by contacting the aqueous solution mixture with a strongly basic ion exchange resin in the chloride form, eluting certain absorbed impurities with an alkali metal salt solution, converting the column to the hydroxide form by contacting the resin with an alkali metal hydroxide solution such that the effluent becomes basic, and eluting absorbed NDP with a weakly acidic alkali metal salt solution such that solutions of highly pure NDP are obtained in basic effluent fractions. The new process is particularly valuable for purification of NDPs directly from chemical synthesis reaction media.

REFERENCES:
patent: 3337529 (1967-08-01), Laufer
patent: 3509128 (1970-04-01), Fujimoto et al.
patent: 3534017 (1970-10-01), Fujimoto et al.
patent: 3787392 (1974-01-01), Bergmeyer et al.
patent: 3803125 (1974-04-01), Bergmeyer et al.
patent: 4430496 (1984-02-01), Abbott
Duolite Ion Exchange Manual, Diamond Shamrock Chemical Co., 1969, cover page, pp. VII, VIII, 96-105, and 162-171.
Michaelson, Biochem. Biophys. ACTA, 1964, 91:1-13.
Moffatt & Corona, J. Amer. Chem. Soc., 1961, 83:649-658.

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