Process for purifying factor VIII:C

Details Process for purifying factor VIII:C Process for purifying factor VIII:C

1983-12-05

1985-04-02

Rosen, Sam

Drug, bio-affecting and body treating compositions

Fermentate of unknown chemical structure

Having a known elemental analysis

A61K 3516

Patent

active

045087091

ABSTRACT:
Factor VIII:C is purified by chromatographic absorption on aminohexyl agarose at a pH above 5.5 up to about 8, and preferably 6.5 to 7.2, and a conductivity of about 25 to about 35 mS/cm.

REFERENCES:
patent: 4361509 (1982-11-01), Zimmerman et al.
Austen, D. E. G., "The Chromatographic Separation of Factor VIII on Aminohexyl Sepharose", British Journal of Hematology, 1979 (43), pp. 669-674.
Morgenthaler, J. J., "Chromatography of Antihemophilic Factor on Diaminoalkane-and Aminoalkane-Derivatized Sepharose", Thromb. Haemostas., 1982, 47(2), pp. 124-127.
Faure, A., et al., "Note, Improved buffer for the chromatographic separation of Factor VIII coagulant", J. Chromatography, 1983 (257), pp. 387-391.
Austen, D. E. G., et al., "Factor VIII Fractionation on Aminohexyl Sepharose with Possible Reduction in Hepatitis B Antigen", Thromb. Haemostas., (1982) 48(1), pp. 46-48.

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