Process for purifying enzymes

Details Process for purifying enzymes Process for purifying enzymes

1983-04-25

1985-12-24

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

435189, 435190, 435195, 435198, 435232, 435233, 435234, 435815, C12N 900, C12N 902, C12N 904, C12N 914, C12N 920, C12N 988, C12N 990, C12N 992

Patent

active

045606617

ABSTRACT:
Disclosed is a process for purifying an enzyme contained in a solution such as cell extract liquor or fermentation culture liquor. The crude enzyme solution is brought into contact with either a strongly acidic cation exchange resin of high porous type or a strongly basic anion exchange resin of high porous type to adsorb the enzyme on the resin. An eluting agent is then passed through the resin to elute out the enzyme as a purified enzyme solution.

REFERENCES:
patent: 2952586 (1960-09-01), Okunuki et al.
Journal of Fermentation Technology, vol. 58, No. 5, pp. 423-429, (1980).
Chemical Abstract vol. 93:234004b, (1980).
Chemical Abstract vol. 97:68552q, (1984).
Fukunaga, Noriyuki, et al., Chemical Abstracts, vol. 88, no. 3, (1978) p. 253, no. 18297d, "Collection of Urokinase".
Fujita, Yoshimasa, et al., Chemical Abstracts, vol. 85, no. 21, (1976) p.384, no. 157977b, "Recovery of Immobilized Glucose...".
M. Suekane, Chemical Abstracts, vol. 98, no.11 (1983), p. 233, no. 85413h, "Immobilization of Glucose Isomerase...".

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