Process for purifying blood clotting factors

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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530381, 530384, 530382, 530383, 530412, 530416, C12N 974, C07K 14745, C07K 118

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active

054459583

ABSTRACT:
A method for at least partially separating vitamin K-dependent blood clotting factors from a mixture containing at least one such factor, e.g. a prothrombin complex concentrate, which comprises adsorption of said mixture on to a chelate of a polyvalent metal immobilized on an inert support, e.g. Cu.sup.2 + -primed Chelating Sepharose, followed by elution to yield one or more fractions enriched in respect of one of said factors.

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Weerasinghe et al. (1985) Biochim. Biophys. Acta 839:57-61.
Porath, J. et al., Nature, 258:598-9 (1975) "Metal chelate affinity chromatography, a new approach to protein fractionation".
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Andersson, L., J. Chromatog., 315:167-174 (1984) "Fractionation of human serum proteins by immobilised metal affinity chromatography".
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Liebman, H. A., et al., PNAS, 82:3879-3883 (1985) "Immunoaffinity purification of factor IX (Christmas factor) by using confomation specific antibodies directed against the factor IX--metal complex".
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