Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Patent
1993-03-08
1995-11-14
Wityshyn, Michael G.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
530412, 530414, 530427, 435 711, 435 712, 435 695, 424 851, C07K 1453, C07K 114, C07K 134, C12P 2102
Patent
active
054667810
ABSTRACT:
A process is described for producing M-CSF from bacteria. It includes: fermentation of bacteria containing M-CSF DNA; harvest of the fractions that contain the M-CSF protein (refractile bodies); primary recovery of the protein; solubilization and denaturation of refractile bodies; M-CSF refolding; purification by column chromatography and other methods; and formulation of the properly refolded M-CSF. This method is advantageous over prior methods in terms of yield and purity.
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patent: 4929700 (1990-05-01), Halenbeck et al.
patent: 5248769 (1993-09-01), Darin
Yamanishi et al. "Renaturation, Purification & Characterization of Human Truncated MLSE Expressed in Escherichia coli" J. Biochem 404-409 1991.
Remingtons Pharmaceutical Scienes 17th Ed. 1985 pp. 1538-1539 "Freeze Drying".
Halenbeck et al "Purification & Characterization of Recombinant Human M-CSF & Generation of a Neutralizing Ab Useful for Western Analysis". J Biotechnol. 8:45-58 1988.
Halenbeck et al. "Renaturation & Purification of Biologically Active Recombinant Human MCSF Expressed in E coli Bio Technology". 7:(7) 1989 pp. 710-715.
Chang Byeong S.
Cowgill Cynthia A.
Dorin Glenn
Gray David R.
Milley Robert J.
Blackburn Robert P.
Chiron Therapeutics
Chung Ling-Fong
Degen Nancy J.
McGarrigle, Jr. Philip L.
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