Process for purifying and stabilizing catechol-containing protei

Details Process for purifying and stabilizing catechol-containing protei Process for purifying and stabilizing catechol-containing protei

1984-03-07

1985-01-29

Schain, Howard E.

Compositions: coating or plastic

Coating or plastic compositions

Proteinaceous material containing

106124, 106160, 260112R, A23J 104, C07G 700, C08L 8900, C09J 320

Patent

active

044963971

ABSTRACT:
A process is provided for purifying and stabilizing marine mussel polyphenolic proteins rich in 3,4-dihydroxyphenylalanine (dopa) and hydroxyproline (hyp) while obtaining high yields thereof. The process includes the steps of providing an acid soluble extract of the dopa-containing proteins, removing the low molecular weight material from the extract and reacting the remaining proteinaceous material with a water soluble borate at a pH of 7.0-9.0 to provide a soluble borate complex of the dopa-containing protein while precipitating impurities. The complex is separated from the precipitate and may be concentrated for storage or treated with an acetic acid solution and either concentrated or lyophilized and stored under an inert atmosphere. The purified proteins exhibit a dopa:protein index of purity ratio of at least about 0.10.

REFERENCES:
patent: 2602031 (1952-07-01), Ugelstad
patent: 4390468 (1983-06-01), Sasaki et al.
patent: 4402874 (1983-09-01), Johnson et al.
patent: 4436656 (1984-03-01), Sasaki et al.
patent: 4436657 (1984-03-01), Sasaki et al.
J. of Biol.-Chem. 258, No. 5, pp. 2911-2915, Mar. 10, 1983, Waite.
Science, 212, pp. 1038-1040 (1981), Waite et al.

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