Process for purifying and separating vitamin B.sub.12

Details Process for purifying and separating vitamin B.sub.12 Process for purifying and separating vitamin B.sub.12

1981-11-24

1983-05-10

Chan, Nicky

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C07H 1512

Patent

active

043831109

ABSTRACT:
A process for separating vitamin B.sub.12 in pure form, which comprises bringing a vitamin B.sub.12 -containing liquid containing impurities into contact with a resin selected from the group consisting of (A) a divinylbenzene/styrene copolymer having a mode pore diameter of at least about 200 A and a total pore volume of more than 0.6 ml/g, and (B) a copolymer of divinylbenzene/styrene/an unsaturated alkyl ester of an aromatic polycarboxylic acid represented by the formula ##STR1## wherein R represents a C.sub.3 -C.sub.10 unsaturated alkyl moiety having a carbon-carbon double bond and n is 2 or 3, said copolymer (B) having a surface area of at least about 700 m.sup.2 /g, to cause vitamin B.sub.12 to be adsorbed to said resin, and eluting the vitamin B.sub.12 with an eluent, and collecting the active eluate.

REFERENCES:
The Merck Index, 9th Ed., Merck & Co., Inc., Rahway, N.J., 1976, pp. 1287-1288.

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