Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lipoproteins – e.g. – egg yolk proteins – cylomicrons – etc.
Patent
1997-09-05
2000-08-22
Low, Christopher S. F.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lipoproteins, e.g., egg yolk proteins, cylomicrons, etc.
530412, 530421, 530422, 530424, 435 691, C07K 114, C07K 14775
Patent
active
061074675
ABSTRACT:
The present invention relates to a process for purifying a hydrophobic or amphiphilic compound, by first mixing a starting material containing the hydrophobic or amphiphilic compound with a first polymeric material, water and at least one of a second polymeric material and a surfactant, wherein the first polymeric material and the second polymeric material and/or surfactant are immiscible in the resulting primary aqueous solution. The process further comprises maintaining the primary aqueous solution for a period of time sufficient for essentially separating the phases formed, and then removing the phase containing the main portion of the hydrophobic or amphiphilic compound and the second polymeric material and/or surfactant. The second polymeric material and/or surfactant are separated from the hydrophobic or amphiphilic compound, and subsequently recycled to the initial mixing step. The present invention further relates to a composition for use in purification of apolipoprotein A (ApoA) or apolipoprotein E (ApoE), said composition comprising a first polymeric material and a surfactant, said first polymeric material and surfactant being immiscible in the primary aqueous solution obtained after mixing with water. ApoA or ApoE produced by the inventive process can be used for the manufacture of a medicament in the treatment of atherosclerosis and cardiovascular diseases, sepsis or peripheral atherosclerosis as well as in a method for treatment of atherosclerosis and cardiovascular diseases, sepsis or peripheral atherosclerosis when administered in a therapeutically effective amount.
REFERENCES:
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Harris et al. `Enzyme Purification Using Temperature Induced Phase Formation`, Bioseparation vol. 2, pp. 237-246, 1991.
Calabresi et al. `Molecular Characterization of Native and Recombinant Apolipoprotein A-I Milano Dimer`, J. of Biol. Chem. vol. 269, No. 23, pp. 32168-32174, Dec. 23, 1994.
Database Caplus on STN. No. 1995:421793. Soma et al. `Recombinant Apolipoprotein A-Imilano Dimer Inhibits Carotid Intimal Thickening Induced by Pervascular Manipulation in Rabbits`, Cir. Res. vol. 76, No. 3, pp. 405-411, 1995.
Ageland Hans
Nystrom Lena
Persson Josefine
Tjerneld Folke
Gupta Anish
Low Christopher S. F.
Pharmacia & UpJohn AB
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