Process for purification of enzymes

Details Process for purification of enzymes Process for purification of enzymes

1985-05-22

1987-05-05

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Isomerase

435814, C12N 992

Patent

active

046632883

ABSTRACT:
The disclosure is directed to the resolubilization of an insoluble glucose isomerase-amine complex, wherein the amine has the general formula: ##STR1## The insoluble enzyme complex may be resolubilized to produce a stable concentrated and purified glucose isomerase preparation by reaction with a resolubilizing mixture comprising a cation exchange resin.

REFERENCES:
patent: 3728324 (1973-04-01), Borglum
patent: 3769168 (1973-10-01), Masuda
patent: 3912595 (1975-10-01), Philipp et al.
patent: 3972777 (1976-08-01), Yamada et al.
patent: 4055469 (1977-10-01), Snoke et al.
patent: 4106992 (1978-08-01), Vairel et al.
patent: 4144130 (1979-03-01), Kula et al.
patent: 4347322 (1982-08-01), Johnson et al.

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