Process for purification of basic fibroblast growth factor

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.

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530412, 530416, 530417, C07K 322, C07K 320, C07K 1300

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active

053310950

ABSTRACT:
The invention relates to a method of purifying basic fibroblast growth factor. The method involves the use of strong cation exchange chromatography followed by hydrophobic interaction chromatography and then by weak cation exchange chromatography.

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Barr et al., "Expression and processing of biologically active fibroblast growth factors in the yeast Saccharomyces cerevisiae" J. Biol. Chem. (1988) 263(31):16471-16478.
Fox et al., "Production, biological activity, and structure of recombinant basic fibroblast growth factor and an analog with cysteine replaced by serine" J. Biol. Chem. (1988) 263:18452-18458.
Squires et al., "Production and characterization of human basic fibroblast growth factor from Escherichia coli" J. Biol. Chem. (1988) 263(31):16297-16302.

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