Process for proteolytic cleavage and purification of...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

Reexamination Certificate

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C435S069700, C435S069100, C800S278000, C530S413000

Reexamination Certificate

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07834161

ABSTRACT:
The present invention relates to improved methods for protein purification of high-value heterologous proteins by providing fusion proteins suitable for affinity purification and improved and economical methods of proteolytic cleavage of fusion proteins. The methods are useful for large-scale production of purified recombinant proteins from plants, plant-derived tissue or plant cells. The invention aims to reduce the cost and improve the quality of downstream processing of heterologous proteins produced in plants and other biological production systems.

REFERENCES:
patent: 6048715 (2000-04-01), Haynes et al.
patent: 7462701 (2008-12-01), Mantyla et al.
patent: 2002/0164718 (2002-11-01), Tchaga et al.
patent: WO-00/77174 (2000-12-01), None
patent: WO-02/05922 (2002-01-01), None
Kisselev L., Polypeptide release factors in prokaryotes and eukaryotes: same function, different structure. Structure, 2002, vol. 10: 8-9.
Wishart et al., A single mutation converts a novel phosphotyrosine binding domain into a dual-specificity phosphatase. J. Biol. Chem., 1995, vol. 270(45): 26782-26785.
Witkowski et al., Conversion of a b-ketoacyl synthase to a malonyl decarboxylase by replacement of the active-site cysteine with glutamine. Biochemistry, 1999, vol. 38: 11643-11650.
Boraston, Alisdair B et al., Protein Expression and Purification, vol. 21, No. 3, Apr. 2001, pp. 417-423.
Boraston, Alisdair B et al., Biochemistry, vol. 40, No. 21, May 29, 2001, pp. 6240-6247.
Reeves, R.A. et al., Applied and Environmental Microbiology, Apr. 2000, vol. 66, No. 4, pp. 1532-1537.
Chhabra, S.R. et al., FEBS Letters, Elsevier Science Publishers, vol. 531, No. 2, Nov. 6, 2002, pp. 375-380.

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