Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2005-11-22
2005-11-22
Weber, Jon (Department: 1653)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C530S413000, C530S416000, C530S422000, C530S350000, C435S007100, C435S325000, C435S252100, C435S252300, C435S252330, C424S487000, C424S488000
Reexamination Certificate
active
06967241
ABSTRACT:
The invention includes a process for extracting a target protein fromE. colicells that includes lowering the pH of a wholeE. colicell solution to form an acidic solution, disrupting the cells to release the protein into the acidic solution, and separating the cellular debris from the released protein to obtain a protein product enriched in the heterologous target protein. The invention also includes addition of a solubility enhancer.
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Wheelwright, S., “Protein Purification, Design and Scale up of Downstream Processing,” Chapter 5 and 6, p. 52-81, 1991, John Wiley & Sons Inc, New York.
Genentech Inc.
Robinson Hope
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