Process for production of secretory Kex2 derivatives

Details Process for production of secretory Kex2 derivatives Process for production of secretory Kex2 derivatives

1997-03-04

1999-03-23

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4352542, 536 232, C12N 960, C12N 119, C12N 1557

Patent

active

058858210

ABSTRACT:
Kex2 protease derivatives obtained by transforming methanol-assimilating with expression vectors containing DNA coding for any amino acid sequence which is an amino acid sequence of Kex2 protease wherein the N-terminus is the Met at position 1 and the C-terminus is one of the amino acids between positions 618 (inclusive) and 698 (inclusive), or a modification of that amino acid sequence, culturing the resulting transformants and recovering the derivatives from the cultures, as well as gene systems coding for the derivatives and a method for producing the Kex2 protease derivatives using the gene systems. Also, a method for excision of desired peptides using the Kex2 protease derivatives.

REFERENCES:
patent: 5162220 (1992-11-01), Oshima et al.
Charles Brenner et al., "Structural And Enzymatic Characterization Of A Purified Prohormone-Processing Enzyme; Secreted, Soluble Kex2 Protease", Proc. Nat'l. Academy Sci. USA, vol. 89, pp. 922-926, (1992).
Doris Germain et al., "Expression Of The Saccharomyces Cerevisiae Kex2p Endoprotease In Insect Cells Evidence For A Carboxy-Terminal Autoprocessing Event", Eur. J. Biochem, vol. 204, 121-126 (1992).
Peter G. Seebooth, "In-vitro Cleavage Of a Fusion Protein Bound To Cellulose Using The Soluble yscFs (Kex2) Variant", Appl. Microbiol Biotechnol, vol. 37, 621-625, (1992).

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