Process for production of exogenous gene or its product in plant

Details Process for production of exogenous gene or its product in plant Process for production of exogenous gene or its product in plant

1993-04-28

1995-05-23

Fox, David T.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 691, 435 701, 4352351, 4353201, 536 232, 536 2372, C12N 1500, C12N 700, C12P 2104, C07H 2104

Patent

active

054181534

ABSTRACT:
A process for production of an exogenous gene or its product in a plant cell which comprises:
inserting into a genome of a plant;
a) cDNA of replicase gene from RNA plant virus, and
b) cDNA of a recombinant virus genomic RNA in which nucleotide moiety at and after ATG downstream from the original translation initiation codon (the first ATG counted from the 5'-end) in the cDNA of coat protein gene is replaced with a desired exogenous gene; or
inoculating a plant cell including cDNA of replicase gene of a plant virus with synthesized RNA synthesized from the cDNA of recombinant virus genomic RNA.

REFERENCES:
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Haseloff et al. 1984. Proc. Natl. Acad. Sci. USA 81: 4358-4362.
Van Dun et al. 1988. Virology 163: 572-578.
Journal Of General Virology, vol. 73, 1992, pp. 169-172, M. Mori et al, "Expression of brome mosaic virus-encoded replicase genes in transgenic tobacco plants".
Science, vol. 231, 1986, R. French et al, "Bacterial Gene Inserted in an Engineered RNA Virus; Efficient Expression in Monocotyledonous Plant Cells", pp. 1294-1297.
The EMBO Journal, vol. 6, 1987, N. Takamatsu et al, "Expression of bacterial acetyltransferase gene in tobacco plants mediated by TMV-RNA", pp. 307-311
Annual Review Of Phytopathology, vol. 28, 1990, pp. 451-474, R. N. Beachy et al, "Coat Protein-Mediated Resistance Against Virus Infection".

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