Process for production of diphtheria toxin

Details Process for production of diphtheria toxin Process for production of diphtheria toxin

2007-09-13

2009-08-18

Navarro, Mark (Department: 1645)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Reexamination Certificate

active

07575891

ABSTRACT:
The present invention provides a method of purifying diphtheria toxin comprising (1) fermenting a microorganism strain capable of producing diphtheria toxin using glucose as a carbon source, said method comprising adding glucose to a growing culture whereby the addition of glucose maintains microorganism growth effective to support diphtheria toxin production; and (2) purifying the diphtheria toxin from the culture by contacting a toxin containing preparation derived therefrom with an ion exchange matrix, eluting a fraction containing the toxin, applying the eluate to a hydrophobic matrix, and eluting a fraction containing the toxin.

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Rappuoli, et.al. “Rapid Purification of Diphtheria Toxin by Phenyl Sepharose and DEAE Cellulose Chromatography” Journal of Chromatography, vol. 268, 1983, pp. 543-548.
Wen, et.al. “Diphtheria Toxin-Related Alpha-Melanocyte-Stimulating Hormone Fusion Toxin” The Journal of biological Chemistry, vol. 266, No. 19, Jul. 5, 1991, pp. 12289-12293.
Zhidkova (Tr. Irkutskogo Nauchn.-Issled. Inst. Epidemiol. I Gigieny (1960) No. 5 pp. 73-76.

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