Process for producing vitamin B 12 from...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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Reexamination Certificate

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06972188

ABSTRACT:
Mesophilic methane bacteria obtained from digested sludge are acclimatized in an H2/CO2medium and the acclimatized methane bacteria are grown on a support inclusive of inorganic nutritional salts of trace metal elements by using an immobilized bed bioreactor. The grown bacteria are allowed to metabolize a mixed gas, prepared by adding hydrogen to at least one of coal gas and biogas, into methane and at the same time, cobalamin contained in the fermented liquor is recovered as an extracellular product in the form of cyanocobalamin by using potassium cyanide to thereby produce vitamin B12efficiently in a high content and high yield.

REFERENCES:
patent: 881287 (1998-12-01), None
patent: 2-286093 (1990-11-01), None
patent: 4-4892 (1992-01-01), None
patent: 4-23979 (1992-01-01), None
patent: 6-169783 (1994-06-01), None

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