Process for producing trypsin (trypsinogen)

Details Process for producing trypsin (trypsinogen) Process for producing trypsin (trypsinogen)

1997-10-22

1999-08-31

Jacobson, Dian C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

43525411, 4352543, 4353201, 536 232, C12N 115, C12N 976, C12N 1555, C12N 1580

Patent

active

059453281

ABSTRACT:
Trypsin (trypsinogen) may be produced in a filamentous fungus by transforming a filamentous fungus with a vector comprising a DNA sequence encoding protrypsin or a derivative thereof N-terminally fused to a DNA sequence encoding a signal peptide, culturing the transformed filamentous fungus in a suitable culture medium to produce trypsinogen and recovering trypsinogen and/or trypsin from the medium.

REFERENCES:
patent: 5536661 (1996-07-01), Boel
patent: 5679543 (1997-10-01), Lawlis
Emi, M. et al. Gene 41:305-310 (1986).
Rypniewski, W.R. Protein Engineering 7(1):57-64 (1994).

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