Process for producing the enzyme cholesterol esterase and for hy

Details Process for producing the enzyme cholesterol esterase and for hy Process for producing the enzyme cholesterol esterase and for hy

1981-07-13

1983-06-07

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 11, 435824, C12N 916, C12Q 160, C12R 1025

Patent

active

043871631

ABSTRACT:
The enzyme cholesterol esterase is produced by cultivating a strain of the Achromobacter delicatulus type, isolated from vegetable debris and identified by the symbol NRRL B-12115. Under the described culture conditions, the enzyme is exocellular and therefore does not require extraction from the cells.
In addition, the micro-organism has a duplication time less than that of moulds and Actinomycetes. Cholesterol esters of fatty acids can be hydrolysed by adding them to the culture medium before inoculating the strain.

REFERENCES:
patent: 3925164 (1975-12-01), Beaucamp et al.
patent: 4011138 (1977-03-01), Terada et al.
patent: 4042461 (1977-08-01), Esders et al.
patent: 4052263 (1977-10-01), Masurekar et al.

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