Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving blood clotting factor
Reexamination Certificate
2007-09-18
2007-09-18
Wax, Robert A. (Department: 1656)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving blood clotting factor
C514S002600, C530S300000
Reexamination Certificate
active
09926256
ABSTRACT:
It is provided a method for producing a subunit peptide originating from an oligomeric protein having disulfide bonds within a subunit and between subunits. The method comprises producing a subunit originating from an oligomeric protein having disulfide bonds within a subunit and between subunits by the following steps:(a) a step of refolding the subunit by denaturing the oligomeric protein or its subunit in a solution with a protein-denaturing agent and removing the denaturing agent from the solution in the presence of polyoxyalkyl polyether having a functional group that reacts with a thiol group; and(b) a step of isolating the subunit bonded to the polyoxyalkyl polyether from the solution.
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Fukuchi Naoyuki
Kageyama Shunsuke
Kayahara Takashi
Kito Morikazu
Yamamoto Hiroshi
Ajinomoto Co. Inc.
Desai Anand
Oblon & Spivak, McClelland, Maier & Neustadt P.C.
Wax Robert A.
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