Process for producing restriction enzyme

Details Process for producing restriction enzyme Process for producing restriction enzyme

1985-10-03

1988-02-09

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4351721, 435 6, 435822, 935 77, C12N 922, C12N 1500, C12Q 168, C12R 101

Patent

active

047242094

ABSTRACT:
A process for producing a restriction endonuclease capable of recognizing the nucleotide sequence ##STR1## (wherein A, C, G and T represent adenosine, cytidine, guanosine and thymidine, respectively) on a DNA chain and specifically cleaving the double-stranded chain at the arrow-marked positions. This process comprises growing a microorganism belonging to the genus Halococcus and capable of producing said restriction endonuclease, and collecting the enzyme thus formed from the culture broth.

REFERENCES:
Roberts, Nucleic Acid Research, vol. 11, No. 1, pp. r135-r167, (1983).

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